IPB Researchers Created Isinglass from Patin’s Swim Bladders
Isinglass is a protein collagen-based product resulted by utilizing fish swim bladder or fish skin section. Isinglass has several functions. One of the functions is clarifier/fining materials on fermented beverage products.
Three researchers from the Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University (FPIK) namely Nurjanah, Wini Trilaksani and Herlan Widya Utama conducted an experiment to learn how to make isinglass by Patin fish (Pangasius hypophthalmus) swim bladder and to study isinglass through determination of proteins, water-soluble proteins, moisture content, pH and its effectiveness as a purifier by measuring the color components in the value scales tested on juice or citrus juice.
The raw material used in this research was swim bladders from Patin fish (Pangasius hypophthalmus) obtained from Fish Production Laboratory and Fishery Field Laboratory, IPB, Bogor.
From the experiment, it was known that the largest content of isinglass is protein, while the water soluble protein content is low. The amount of protein contained in sample A (3 percent isinglass solution) was 94.61 percent, sample B (1.5 percent isinglass solution) of 94.38 percent, and sample C (one percent isinglass solution) of 94.63 percent. The amount of water soluble protein contained in dry isinglass samples for sample A was 17.07 percent, sample B was 15.01 percent, and sample C was 12.52 percent. The higher the protein and the lower the water-soluble protein contained by the isinglass, the better the activity of isinglass as a fining agent.
"Swim bladders used as raw materials for isinglass should be from a very fresh fish that just reached the stage of rigor mortis, so its chemical component has not yet through decomposition stage and its functional properties can still be maintained. The role of temperature is big enough in determining the required time for fish to enter, through and pass the pre-rigor, rigor, and post-rigor.The lower the temperature of fish handling after captured, the slower the fish enter the rigor stage and the longer the time it ends, "said Nurjanah.
The swim bladder of Patin fish has a thick internal membrane so it is very potential to be processed into isinglass. Isinglass is a derivative of insoluble collagen protein in water. Proximate analysis showed the main components of the first isinglass were crude protein about 77.52 percent, water-soluble protein about 12.36 percent, water about 17.75 percent, ash about 0.41 percent, and fat about 0.3 percent. Isinglass has a high acidity of about 2.8 so it has good durability. Based on the measurement of color density using the Tintometer tool, isinglass capability as the best fining agent is owned by samples B and C. (TK)
